leuconostoc dextranicum strain atcc 19255  (ATCC)

Journal:

Article Title: Identification of a Replicon from pTXL1, a Small Cryptic Plasmid from Leuconostoc mesenteroides subsp. mesenteroides Y110, and Development of a Food-Grade Vector

doi: 10.1128/AEM.68.12.6451-6456.2002

Figure Lengend Snippet: Bacterial strains and plasmids

Article Snippet: Bacterial strains and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Relevant characteristic(s) Source or reference a Strains Escherichia coli DH5α recA endA1 gyrA96 thi-1 hsdR17 supE44 Δ lac U169(φ80 Δ lacZ ΔM15) deoR F − λ − Gibco-BRL Leuconostoc mesenteroides subsp. mesenteroides Y105 Wild-type strain 18 Y110 Wild-type strain CNCM I-1936 a FR52 Wild-type strain 24 Leuconostoc mesenteroides subsp. dextranicum DSM20484 Wild-type strain DSMZ b Leuconostoc cremoris LC Wild-type industrial strain Rhodia Food Lactococcus lactis IL1403 Lab strain 8 MG1363 Lab strain 13 RD230 Wild-type industrial strain Rhodia Food Lactobacillus sake 23K Plasmid cured 3 Listeria ivanovii BUG 497 Lab strain Pasteur Institute Pediococcus acidilactici P120 Wild-type industrial strain Rhodia Food Plasmids pBSSKII+ pBluescript SK II+ cloning vector, 2.96 kb, Ap r lacZ Stratagene pGhost9:IS S1 pWV01 derivative replicon 23 pFR18 1.8-kb cryptic plasmid from L. mesenteroides FR52 5 pFBYC018 pBSSKII+::1.8-kb Hin dIII pFR18, 4.8 kb, Ap r Em s 5 pFBYC018E pFBYC018::1.1-kb Bam HI pGhost9:IS S1 (Em r ), 5.9 kb, Ap r Em r 5 pFBYC18E pFBYC018E with 2.5-kb Pvu II pBSSKII+ deleted, 3.5 kb, Em r 5 pTXL1 2.66-kb cryptic plasmid from L. mesenteroides Y110 5 pFBYC050 pBSSKII+::2.66-kb Sal I pTXL1, 5.62 kb, Ap r Em s This study pFBYC051 pBSSKII+::2.66-kb Spe I pTXL1, 5.62 kb, Ap r Em s This study pFBYC052 pBSSKII+::1.8-kb Ssp I pFR18, 4.8 kb, Ap r Em s This study pFBYC050E pFBYC050::1.1-kb Bam HI pGhost9:IS S1 , 7.72 kb, Ap r Em r This study pFBYC50E pFBYC050E with 2.5-kb Pvu II fragment deleted, Em r This study pFBYC065 pFBYC050E with 312-kb ExoIII c fragment deleted, Ap r Em r This study pFBYC060K pFBYC050E with 482-kb Kpn I- Kpn I fragment deleted, Ap r Em r This study pFBYC062S pFBYC050E with 1.4-kb Sal I- Ssp I fragment deleted, Ap r Em r This study pFBYC068S pFBYC050E with 1.9-kb Sal I- Afl III fragment deleted, Ap r Em r This study pFBYC051E pFBYC051::1.1-kb Bam HI pGhost9:IS S1 , 7.72 kb, Ap r Em r This study pFBYC06 pRW5.6 with Hin dIII deleted 4 pFBYC069 pFBYC051E::669-bp P59, dvnA fragment from pFBYC06 This study pFBYC070 pFBYC069::485-bp dvnA::mesY , mesI PCR product This study Open in a separate window a CNCM, Collection Nationale de Cultures de Microorganisms, France. b DSMZ, Deutsche Sammlung von Mikroorganismen and Zellkulturen, Germany. c ExoIII, exonuclease III.

Techniques: Plasmid Preparation, Clone Assay

Journal:

Article Title: Identification of a Replicon from pTXL1, a Small Cryptic Plasmid from Leuconostoc mesenteroides subsp. mesenteroides Y110, and Development of a Food-Grade Vector

doi: 10.1128/AEM.68.12.6451-6456.2002

Figure Lengend Snippet: Bacteriocin activity assays against Listeria ivanovii BUG 497 overlaying Leuconostoc strains. (A) L. mesenteroides subsp. mesenteroides Y105 (a); nonrecombinant L. mesenteroides subsp. dextranicum DSM20484 (b), and nonrecombinant L. cremoris LC (c); (B) L. mesenteroides subsp. mesenteroides Y105; (a) Leuconostoc mesenteroides subsp. dextranicum DSM20484 containing pFBYC070 (b), and Leuconostoc cremoris LC containing pFBYC070 (c).

Article Snippet: Bacterial strains and plasmids used in this study are listed in Table . table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Relevant characteristic(s) Source or reference a Strains Escherichia coli DH5α recA endA1 gyrA96 thi-1 hsdR17 supE44 Δ lac U169(φ80 Δ lacZ ΔM15) deoR F − λ − Gibco-BRL Leuconostoc mesenteroides subsp. mesenteroides Y105 Wild-type strain 18 Y110 Wild-type strain CNCM I-1936 a FR52 Wild-type strain 24 Leuconostoc mesenteroides subsp. dextranicum DSM20484 Wild-type strain DSMZ b Leuconostoc cremoris LC Wild-type industrial strain Rhodia Food Lactococcus lactis IL1403 Lab strain 8 MG1363 Lab strain 13 RD230 Wild-type industrial strain Rhodia Food Lactobacillus sake 23K Plasmid cured 3 Listeria ivanovii BUG 497 Lab strain Pasteur Institute Pediococcus acidilactici P120 Wild-type industrial strain Rhodia Food Plasmids pBSSKII+ pBluescript SK II+ cloning vector, 2.96 kb, Ap r lacZ Stratagene pGhost9:IS S1 pWV01 derivative replicon 23 pFR18 1.8-kb cryptic plasmid from L. mesenteroides FR52 5 pFBYC018 pBSSKII+::1.8-kb Hin dIII pFR18, 4.8 kb, Ap r Em s 5 pFBYC018E pFBYC018::1.1-kb Bam HI pGhost9:IS S1 (Em r ), 5.9 kb, Ap r Em r 5 pFBYC18E pFBYC018E with 2.5-kb Pvu II pBSSKII+ deleted, 3.5 kb, Em r 5 pTXL1 2.66-kb cryptic plasmid from L. mesenteroides Y110 5 pFBYC050 pBSSKII+::2.66-kb Sal I pTXL1, 5.62 kb, Ap r Em s This study pFBYC051 pBSSKII+::2.66-kb Spe I pTXL1, 5.62 kb, Ap r Em s This study pFBYC052 pBSSKII+::1.8-kb Ssp I pFR18, 4.8 kb, Ap r Em s This study pFBYC050E pFBYC050::1.1-kb Bam HI pGhost9:IS S1 , 7.72 kb, Ap r Em r This study pFBYC50E pFBYC050E with 2.5-kb Pvu II fragment deleted, Em r This study pFBYC065 pFBYC050E with 312-kb ExoIII c fragment deleted, Ap r Em r This study pFBYC060K pFBYC050E with 482-kb Kpn I- Kpn I fragment deleted, Ap r Em r This study pFBYC062S pFBYC050E with 1.4-kb Sal I- Ssp I fragment deleted, Ap r Em r This study pFBYC068S pFBYC050E with 1.9-kb Sal I- Afl III fragment deleted, Ap r Em r This study pFBYC051E pFBYC051::1.1-kb Bam HI pGhost9:IS S1 , 7.72 kb, Ap r Em r This study pFBYC06 pRW5.6 with Hin dIII deleted 4 pFBYC069 pFBYC051E::669-bp P59, dvnA fragment from pFBYC06 This study pFBYC070 pFBYC069::485-bp dvnA::mesY , mesI PCR product This study Open in a separate window a CNCM, Collection Nationale de Cultures de Microorganisms, France. b DSMZ, Deutsche Sammlung von Mikroorganismen and Zellkulturen, Germany. c ExoIII, exonuclease III.

Techniques: Activity Assay